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KMID : 1188320160100030348
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Gut and Liver
2016 Volume.10 No. 3 p.348 ~ p.355
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Gastroprotective Effects of PMK-S005 against Ethanol-Induced Acute Gastric Damage in Rats
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Choi Yoon-Jeong
Kim Na-Young
Lee Ju-Yup
Nam Ryoung-Hee
Seo Ji-Hyung
Lee Seon-Min
Kim Hee-Jin
Choi Yoon-Jin
Lee Hye-Seung
Lee Dong-Ho
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Abstract
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Background/Aims:This study aimed to examine the gastroprotective effects of PMK-S005, which is a synthetic S-allyl-l-cysteine (SAC; a sulfur-containing amino acid), against acute ethanol-induced gastric damage in rats.
Methods:Sprague-Dawley rats were divided into six groups, including a nonethanol group, groups treated with absolute ethanol 1 hour after pretreatment with various doses of PMK-S005 (1, 5, and 10 mg/kg) or rebamipide (50 mg/kg), and an absolute ethanol-only group. Ethanol-induced gross ulcer and mucus levels were measured. Myeloperoxidase, tumor necrosis factor α, interleukin 1β, PGE2, LTB4, cPLA2, COX-1, and COX-2 levels were estimated by enzyme-linked immunosorbent assay or Western blot analysis. Furthermore, the protein expression levels of antioxidant enzymes, including heme oxygenase-1 (HO-1), NAD(P)H:quinine oxidoreductase 1 (NQO-1), GCLC, and GCLM, were assessed.
Results:PMK-S005 significantly attenuated the ethanol-induced gastric damage; it reduced mucosal inflammatory cytokine production and increased mucus levels. The expression levels of cPLA2, COX-1, and COX-2 were decreased by PMK-S005. PMK-S005 did not affect PGE2 synthesis, but LTB4 production was significantly suppressed. In addition, long-term administration of PMK-S005 significantly increased the expression of HO-1, NQO-1, GCLC, and GCLM.
Conclusions:These results strongly suggest that PMK-S005 prevents gastric mucosal damage and that these gastroprotective activities are due to anti-inflammatory effects and enhancement of the gastric defense system, including antioxidant enzymes
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KEYWORD
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S-allyl--cysteine, Gastroprotection, Anti-inflammation, Antioxidants, Ethanol
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